摘要：Background Some hyperthermia studies in oncology deals with connected immune-effects. Modulated electro-hyperthermia (oncothermia, Szasz et al. (2010) [1]) is an emerging curative treatment method. It is selectively focused in the malignant cells without harming the non-malignant surrounding tissues Andocs et al. (2009) [2]. Aim Our objective is studying the cell-death process caused by the treatment. Method HT29 human colorectal xenograft and C26 mouse colorectal allograft models were studied in vivo, on in time-course investigations. We apply 13.56 MHz radiofrequency (RF) signal modulated by 1/f noise pattern. In silico models were performed visualising the selection process. Samples were analysed by various histomorphological and immunhistochemical analyses. Results A massive TUNEL positivity was produced. Up regulation of TRAILR2 (DR5), FAS and FADD was observed. AIF nuclear translocalisation together with mitochondrial pore formation and cytochrome-C release, as well as DNA fragmentation was measured. A damage associated molecular pattern (DAMP) was formed, which are probably parts of the immunogenic cell-death (ICD) of the selected malignant cells. Conclusion The DAMP associated, induced ICD can be a good basis for hyperthermia and immunotherapy combination.