Hypoxia inducible factor 1α down regulates cell surface expression of α1,2-fucosylated glycans in human pancreatic adenocarcinoma cells
摘要：Abstract The α1,2-fucosyltransferase activity in pancreatic tumors is much lower compared to normal pancreatic tissue. Here we show that hypoxia inducible factor (HIF) 1α is constitutively expressed in the pancreatic cancer cell lines Pa-Tu-8988S and Pa-Tu-8988T and suppresses the expression of the α1,2-fucosyltransferase genes FUT1 and FUT2. Down regulation of HIF-1α expression resulted in elevated FUT1 and FUT2 transcript levels and an increased expression of α1,2-fucosylated glycan structures on the surface of these cells. In conclusion, our data are the first to identify HIF-1α as a suppressor of FUT1/2 expression, thereby regulating α1,2-fucosylation of cell-surface glycans.
关键词：HIF hypoxia-inducible factor; hypoxia-inducible factor; Ley Lewis Y; Lewis Y; Lex Lewis X; Lewis X; BG blood group; blood group; PDAC pancreatic ductal adenocarcinoma; pancreatic ductal adenocarcinoma; PC pancreatic cancer; pancreatic cancer; ARNT arylhydrocarbon receptor nuclear transporter; arylhydrocarbon receptor nuclear transporter; UEA-1 Ulex europaeus agglutinin I; Ulex europaeus agglutinin I; PaTu-S Pa-Tu-8988S; Pa-Tu-8988S; PaTu-T Pa-Tu-8988T; Pa-Tu-8988T; HRE hypoxia response element; hypoxia response element; iRNA interfering RNA; interfering RNA; KD knock down; knock down; Abs antibodies; antibodies; HRP Horseradish peroxidase-conjugated; Horseradish peroxidase-conjugated; RT room temperature; room temperature; Glycosyltransferase; Regulation of gene expression; Fucosyltransferase; Hypoxia inducible factor 1 α (HIF-1α); Cancer
论文方向：[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊：FEBS Letters Volume 589, Issue 18
发表时间：Wed Aug 19 00:00:00 CST 2015
数字识别码：10.1016/j.febslet.2015.07.035
是否作者本人： 否
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