Fluorescence digital image analysis of the inositol trisphosphate-mediated calcium transient in single permeabilized parietal cells
摘要：Abstract The myo-inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ mobilization in single saponin-permeabilized and fura-2-loaded parietal cells was analysed by a fluorescence digital image processor. When the cells were incubated with ATP, free cytoplasmic Ca2+ concentration ([Ca2+]i) increased in some restricted cytoplasmic regions showing discontinuous plateau and in the peripheral cytoplasm showing continuous [Ca2+]i gradient towards the plasma membranes. When treated with IP3, the high plateau enlarged to the entire cytoplasm and (a) new higher plateau(s) appeared and enlarged again in a transient manner. The IP3-induced Ca2+ transient was also observed by fluorescence microphotometry of the single cells or by fluorescence spectrophotometry and 45Ca2+ uptake experiment of the cell population.
关键词：Fluorescence digital imaging; Ca2+ transient; Inositol trisphosphate; Permeabilized cell; (Parietal cell, Guinea pig); fura-2, 1,(2-(5′-carboxyoxazol-2′-yl)-6-amino-benzofuran-5-oxy)-2-(2′-amino-5′-methylpheoxy)ethane-N,N,N′,N′-tetraacetic acid; Mops, 3-(N-morpholino)propanesulfonic acid
论文方向：[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊：FEBS Letters Volume 231, Issue 1
发表时间：Mon Apr 11 00:00:00 CDT 1988
数字识别码：10.1016/0014-5793(88)80696-3
是否作者本人： 否
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