Direct photoaffinity labeling of the putative sulfonylurea receptor in rat β-cell tumor membranes by [3H]glibenclamide
摘要：Abstract The oral antidiabetic sulfonylurea [3H]glibenclamide specifically binds to plasma membranes from a rat β-cell tumor indicating a receptor for sulfonylureas in these membranes. Irradiation of [3H]glibenclamide at 254 or 300 nm in the presence of albumin resulted in covalent labeling of the albumin molecule. Direct photoaffinity labeling of β-cell membranes with [3H]glibenclamide resulted in the covalent modification of two membrane polypeptides with apparent molecular masses 140 and 33 kDa. The extent of labeling of the 140 kDa polypeptide was specifically decreased by sulfonylureas. This suggests that a membrane polypeptide of 140 kDa is a component of the sulfonylurea receptor in the β-cell membrane.
关键词：Sulfonylurea receptor; β-Cell membrane; Photoaffinity labeling; Glibenclamide
论文方向：[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊：FEBS Letters Volume 229, Issue 2
发表时间：Mon Mar 14 00:00:00 CST 1988
数字识别码：10.1016/0014-5793(88)81155-4
是否作者本人： 否
版权及免责声明：
本网站所有论文文件均系用户自行上传或提供，本网站对其内容准确性及合法性概不负责，亦不承担任何法律责任。论文版权归原作者及原出处所有。
如您发现网站其他用户上传的论文有侵犯您的姓名权、隐私权、著作权或其他合法权益现象的，请及时与本网站联系并附加相关权利证明文件，以便本网站及时作出处理，维护您的合法权益。
本网站拥有对此声明的最终解释权。
{replyUser1} 回复 {replyUser2}：{content}