Carbohydrate induced modulation of cell membrane. VI. Binding of exogenous lectin induces susceptibility of erythrocytes to free radical damage: a spin label study
摘要：Abstract The oxidation of erythrocyte membrane has been widely used as a model to study the damage of biomembranes by free radicals. Whether binding of lectin to erythrocytes has any effect on peroxidant injury has never been studied. This study reports for the first time that crosslinking of erythrocyte surface glycoprotein by an exogenous lectin significantly enhances the susceptibility to membrane damage by free radicals, as evidenced by the increase in membrane fluidity measured by EPR using spin label and the increase in the amount of oxyhemoglobin liberated due to cell lysis.
关键词：Lectin; Red blood cell membrane; Pyrogallol; Electron paramagnetic resonance; 16-DS 16-doxyl stearic acid; 16-doxyl stearic acid; PBN N-t-butyl-phenyl nitrone; N-t-butyl-phenyl nitrone; PBS 0.01 M phosphate buffer pH 7.4 with 0.15 M NaCl; 0.01 M phosphate buffer pH 7.4 with 0.15 M NaCl; EPR electron paramagnetic resonance; electron paramagnetic resonance
论文方向：[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊：FEBS Letters Volume 406, Issue 3
发表时间：Mon Apr 14 00:00:00 CST 1997
数字识别码：10.1016/S0014-5793(97)00272-X
是否作者本人： 否
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