The death domain protein p84N5, but not the short isoform p84N5s, is cell cycle-regulated and shuttles between the nucleus and the cytoplasm
摘要：Abstract P84N5 is a death domain containing protein that interacts with the tumor suppressor retinoblastoma protein and induces apoptosis. We cloned and characterized two novel alternatively spliced versions of p84N5. The p84N5 short isoform (p84N5s) lacks the death domain and does not induce apoptosis. We showed that p84N5, but not p84N5s, is cell cycle regulated. We found that p84N5-GFP chimera can rapidly shuttle between the nucleus and the cytoplasm. Taken together, these observations suggest that p84N5 may transmit signals from the nucleus to cytoplasmic effectors.
关键词：p84N5; Death domain; Apoptosis; Nucleus; Alternative splicing; DAPI, 4,6-diamidino-2-phenylindole; GFP, green fluorescent protein; RT-PCR, real time polymerase chain reaction
论文方向：[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊：FEBS Letters Volume 574, Issues 1–3
发表时间：Fri Sep 10 00:00:00 CST 2004
数字识别码：10.1016/j.febslet.2004.07.074
是否作者本人： 否
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