Efficient biochemical engineering of cellular sialic acids using an unphysiological sialic acid precursor in cells lacking UDP-N-acetylglucosamine 2-epimerase
作者:, Stephan Hinderlich
摘要:Abstract Sialic acids comprise a family of terminal sugars essential for a variety of biological recognition systems. N-Propanoylmannosamine, an unphysiological sialic acid precursor, is taken up and metabolized by mammalian cells resulting in oligosaccharide-bound N-propanoylneuraminic acid. N-Propanoylmannosamine, applied to endogenously hyposialylated subclones of the myeloid leukemia HL60 and of the B-cell lymphoma BJA-B, both deficient in UDP-N-acetylglucosamine 2-epimerase, is efficiently metabolized to CMP-N-propanoylneuraminic acid resulting in up to 85% of glycoconjugate-associated sialic acids being unphysiological N-propanoylneuraminic acid. Thus, UDP-N-acetylglucosamine 2-epimerase-deficient cell lines provide an important experimental progress in engineering cells to display an almost homogeneous population of defined, structurally altered sialic acids.
关键词:Biochemical engineering; N-Propanoylmannosamine; Sialic acid; UDP-N-acetylglucosamine 2-epimerase; HPAEC high performance anion exchange chromatography; high performance anion exchange chromatography; FITC fluorescein isothiocyanate; fluorescein isothiocyanate; LFA Limax flavus agglutinin; Limax flavus agglutinin; ManNAc N-acetylmannosamine; N-acetylmannosamine; ManNProp N-propanoylmannosamine; N-propanoylmannosamine; NeuAc N-acetylneuraminic acid; N-acetylneuraminic acid; NeuProp N-propanoylneuraminic acid; N-propanoylneuraminic acid; UDP-GlcNAc 2-epimerase UDP-N-acetylglucosamine 2-epimerase; UDP-N-acetylglucosamine 2-epimerase; VVA Vicia villosa agglutinin; Vicia villosa agglutinin
论文方向:[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊:FEBS Letters Volume 503, Issue 1
发表时间:Fri Aug 10 00:00:00 CST 2001
数字识别码:10.1016/S0014-5793(01)02701-6
是否作者本人: 否
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