1、制备样品细胞的单细胞悬液。2、多聚甲醛固定细胞。3、打孔剂在细胞膜上打孔。4、标记荧光素偶联的抗磷酸化STAT-5抗体。5、流式上样分析。
Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease. Nature Medicine. 2010. 16(2):191-197
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