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2024-11-06 10:53:07
0.1%的DMSO加入淋巴细胞中共培养，会不会对淋巴细胞产生毒性呢？想请教一下各位关于用DMSO溶解sp600125的相关问题。一、sp600125是固体，无法准确称取想要的剂量，如何用DMSO配置一定浓度的母液呢；二、sp600125的保存温度为-20°，而且它属于MAPK抑制剂，而DMSO在常温下才呈现液体状，我将-20°保存的sp600125 加入在室温的DMSO中sp600125 会不会就已经完全变性了呢，整个过程是否需要低温操作呢？如果低温操作，DMSO是固体状，那么还如何配置母液呀？三、0.1%的DMSO加入淋巴细胞中共培养，会不会对淋巴细胞产生毒性呢？ |
3个回答
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SP600125 (molecular weight = 220) is poorly soluble in water. Stock solutions of at least 20 mM can be made by using 100% dimethyl sulfoxide. As a general guide, for every 10 μM SP600125, it is recommended to include 0.1% DMSO in the culture media (e.g., 30 μM/0.3% DMSO). Prewarming of media and the use of serum protein may enhance solubility. SP600125 typically precipitates as fine needles that are visible at 50-fold magnification.The % of DMSO that is cytotoxic to cells is generally 0.1% and above. This does depend upon the cells you are using. ie macrophages more resistant than epithelial cell lines, primary cells are exquisitely sensitive to levels of DMSO |
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这个化合物还稳定的啦，在室温下配好stock solution, 然后分装，用前解冻就可以，反复冻融次数尽量不要太多就可以。DMSO 在细胞培养液中的浓度最好不要超过千分之二。 |
