A PKCβ isoform mediates phorbol ester-induced activation of Erk1/2 and expression of neuronal differentiation genes in neuroblastoma cells
摘要：Abstract Protein kinase C (PKC) activation induces neuronal differentiation of SH-SY5Y neuroblastoma cells. This study examines the role of PKCβ isoforms in this process. The PKCβ-specific inhibitor LY379196 had no effect on 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced neurite outgrowth from SH-SY5Y neuroblastoma cells. On the other hand, PKCβ inhibition suppressed the TPA-stimulated increase in neuropeptide Y mRNA, activation of neuropeptide Y gene promoter elements, and phosphorylation of Erk1/2. The TPA-induced increase in neuropeptide Y expression was also inhibited by the MEK inhibitor PD98059. These data indicate that activation of a PKCβ isoform, through a pathway involving Erk1/2, leads to increased expression of neuronal differentiation genes in neuroblastoma cells.
关键词：Growth-associated protein 43; Mitogen-activated protein kinase; Neuroblastoma cell; Neuronal differentiation; Neuropeptide Y; Protein kinase C; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GAP-43, growth-associated protein 43; NPY, neuropeptide Y; PKC, protein kinase C; TPA, 12-O-tetradecanoylphorbol 13-acetate
论文方向：[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊：FEBS Letters Volume 508, Issue 1
发表时间：Fri Nov 09 00:00:00 CST 2001
数字识别码：10.1016/S0014-5793(01)03043-5
是否作者本人： 否
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