Synchronization of nuclear DNA synthesis in cultured Daucus carota L. cells by aphidicolin
作者:, Silvio Spadari
摘要:Abstract By inhibiting the α-like DNA polymerase, and therefore nuclear DNA synthesis, aphidicolin induces accumulation of suspension cultured carrot cells at the G1/S boundary of the cell cycle. After a 24-h treatment with the drug the accumulation is complete, affecting all the cycling cells (95% of the population). Upon removal of the inhibitor, all cycling cells immediately resume nuclear DNA synthesis and move synchronously throughout the S-phase.
关键词:Aphidicolin; Synchronization; Cultured plant cell; Daucus carota; Nuclear DNA synthesis; DMSO dimethyl-sulfoxide; dimethyl-sulfoxide; SCV ml semidented cells/100 ml culture; ml semidented cells/100 ml culture
论文方向:细胞生物学,生物化学[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}] 发表期刊:FEBS Letters Volume 153, Issue 1
发表时间:Mon Mar 07 00:00:00 CST 1983
数字识别码:10.1016/0014-5793(83)80148-3
是否作者本人: 否
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