Somatostatin and α2-adrenergic agonists selectively inhibit vasopressin-induced cyclic AMP accumulation in MDCK cells
摘要：Abstract The effect of somatostatin and α2-adrenergic agonists on cyclic AMP accumulation was examined in MDCK cells, grown in denned medium. These hormones inhibited vasopressin-induced cyclic AMP formation, without affecting either the basal or the glucagon- and prostaglandin E2-stimulated level. Pretreating the cells with pertussis toxin, or incubating them with MnCl2 at a low concentration reversed the effect of somatostatin and α2-agonists. These results suggest that somatostatin and norepinephrine could selectively modulate the renal effect of vasopressin, via the inhibitory regulatory subunit (Ni) of adenylate cyclase.
关键词：(MDCK cell); cyclic AMP; Vasopressin; Somatostatin; Norepinephrine; Pertussis toxin; SRIF somatotropin release-inhibiting factor (somatostatin); somatotropin release-inhibiting factor (somatostatin); MDCK Madin-Darby canine kidney; Madin-Darby canine kidney; IBMX 3-isobutyl-1-methylxanthine; 3-isobutyl-1-methylxanthine; BSA bovine serum albumin; bovine serum albumin; IAP islet-activating protein (pertussis toxin); islet-activating protein (pertussis toxin); HBSS Hanks' balanced salt solution; Hanks' balanced salt solution
论文方向：[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊：FEBS Letters Volume 198, Issue 1
发表时间：Mon Mar 17 00:00:00 CST 1986
数字识别码：10.1016/0014-5793(86)81180-2
是否作者本人： 否
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