DNA fragmentation is a feature of cystic fibrosis epithelial cells: a disease with inappropriate apoptosis?
摘要：Abstract Cystic fibrosis (CF) is a single-gene disease caused by mutations in the CFTR gene, which result in disrupted chloride secretions with inspissated mucous secretions by exocrine glands. Nick-end labelling was used to assess DNA fragmentation in 14 CF and 24 control duodenal samples, and in two CF and two control lung tissues. In CF small intestine median 46% (range: 30–82) villus enterocytes show DNA fragmentation (vs. 3% (range: 1–7) in controls P<0.001) and median 37.5% (range: 23–79) crypt enterocytes show Ki67 antigen (P<0.001). In CF airways 57% (range: 54–70) of epithelial cells show DNA fragmentation. Inappropriate high DNA fragmentation is a feature of various CF epithelia. This could have great impact in understanding the mechanisms leading to disease.
关键词：Cystic fibrosis; Cystic fibrosis transmembrane conductance regulator; DNA fragmentation; Apoptosis; Programmed cell death; CF cystic fibrosis; cystic fibrosis; CFTR cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator; PCD programmed cell death; programmed cell death; TUNEL terminal deoxynucleotidyl transferase-mediated d-UTP-digoxygenin nick-end labelling; terminal deoxynucleotidyl transferase-mediated d-UTP-digoxygenin nick-end labelling
论文方向：[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊：FEBS Letters Volume 408, Issue 2
发表时间：Mon May 19 00:00:00 CST 1997
数字识别码：10.1016/S0014-5793(97)00347-5
是否作者本人： 否
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