Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment
作者:, Elisabeth Fischer-Friedrich
摘要:Abstract Cell stiffness is a sensitive indicator of physiological and pathological changes in cells, with many potential applications in biology and medicine. A new method, real-time deformability cytometry, probes cell stiffness at high throughput by exposing cells to a shear flow in a microfluidic channel, allowing for mechanical phenotyping based on single-cell deformability. However, observed deformations of cells in the channel not only are determined by cell stiffness, but also depend on cell size relative to channel size. Here, we disentangle mutual contributions of cell size and cell stiffness to cell deformation by a theoretical analysis in terms of hydrodynamics and linear elasticity theory. Performing real-time deformability cytometry experiments on both model spheres of known elasticity and biological cells, we demonstrate that our analytical model not only predicts deformed shapes inside the channel but also allows for quantification of cell mechanical parameters. Thereby, fast and quantitative mechanical sampling of large cell populations becomes feasible.
关键词:
论文方向:细胞生物学,生物物理学[{"id":13,"name":"细胞生物学"},{"id":19,"name":"生物物理学"}] 发表期刊:Biophysical Journal Volume 109, Issue 10
发表时间:Tue Nov 17 00:00:00 CST 2015
数字识别码:10.1016/j.bpj.2015.09.006
是否作者本人: 否
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